This study conducted factorial experiments to compare the impact of increasing amounts of tested forages on degradability and fermentation profiles of seven forages during in vitro incubations with buffered RF inoculum. The experiment involved 7 forages each of 3 amounts (0.1, 0.2, and 0.4 g) for two incubation times (48 and 96 hours). The main effects of F, Am, and F × Am were significant for In vitro dry matter degradability (IVD) and In vitro organic matter degradability (IVOMD) which decreased with increasing amounts of forages. The fermentation profiles varied with the type and amount of forages and time. It was observed that the degradability and fermentation profiles of different forages were influenced by the amount of substrate used during in vitro incubations. However, the changes in IVD and fermentation profiles were not proportionate to the change in forage from 0.1 to 0.4 g. Therefore, the study recommends using 0.4 g substrate for subsequent studies to rank a wide range of feed ingredients.

Author(s) Details:

M. M. H. Khan,
Department of Biochemistry and Chemistry, Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet-3100, Bangladesh.

A. S. Chaudhry,
School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK.

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